Hands-on Flow Cytometry: Learning by Doing! EMBL Course

When:
February 20, 2017 – February 24, 2017 all-day
2017-02-20T00:00:00+01:00
2017-02-25T00:00:00+01:00
Where:
EMBL
Meyerhofstraße 1
69117 Heidelberg
Germany
Cost:
500-700
Contact:
European Molecular Biology Laboratory

Application deadline: 12 December 2016

Course Overview

Flow Cytometry is one of the most powerful single cell analysis methods used throughout the life sciences and medicine. Utilising Flow Cytometry we can identify and quantify different from heterogeneous samples, analyse the corresponding state of proliferation or simply detect the expression of fluorescent reporters. Improvements in assay technology are now enabling scientist to detect mRNAs in cells using flow cytometry.  We will cover the theory of Flow Cytometry and corroborate this information intensively with the practical issues of the most frequent assays as part of our practical course curriculum.

Audience

The course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of Flow Cytometry is of advantage, but not a must.

Modules/Resources

• In-depth principles of Flow Cytometry
• Quality assessment and instrument validation
• Staining of tissues with cell surface antigens
• Staining cell cycle profiles in fixed and live cells
• Assessing proliferation and cell division
• Measuring RNA with FISH
• The beauty and burdens of fluorescence proteins in Flow Cytometry
• Analysing data from Flow Cytometers

Learning Outcomes

Our goal for this course is to teach the basic of this technology with a very big emphasis on practical work. Nothing is better than learning by doing. We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path.